The Investigation of Biochemical and Histological Effects of Ellagic Acid in Experimental Chronic Fluorosis Induced Mice

Objective: The present analysis deals with the effects of ellagic acid treatment and biochemical and histopathological alterations in mice with NaF-induced experimental chronic fluorosis. Materials and Methods: Treatments were carried out on male 28 Swiss albino mice divided into 4 equal groups for 8 weeks. Group I (control) was not applied experimental operation. Group II received subcutaneously (sc) 10 mg/kg/day ellagic acid injection, Group III received per orally 25 mg/L NaF, and Group IV received ellagic acid plus NaF. Levels of lipid bound sialic acid (LSA), malondialdehyde (MDA), paraoxonase (PON) activity and reduced glutathion (GSH) in blood samples were analyzed by spectrophotometric method. The levels of calcium, glucose, total cholesterol, triglyceride, high density lipoprotein (HDL), very low density lipoprotein (VLDL) and low density lipoprotein (LDL) in plasma were measured by autoanalyzer. Following euthanasia, liver and kidney tissues obtained from subjects were evaluated as histopathological. Results: MDA level was significantly higher when levels of GSH and PON activity of group III were lower as compared to other groups. Levels of PON activity and GSH of group IV increased as compared to group III. LSA levels of group III and IV were lower than control group. Conclusion: As a result, in experimental chronic fluorosis was detected to decrease in levels of LSA, GSH, PON activity, calcium and glucose, and increase in MDA level and changes in lipid profiles and cellular degenerations in liver and kidney. Injection of ellagic acid was protective during chronic fluorosis.